Related Experiment Video
Updated: Mar 9, 2026
14:04
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
6.3K
A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy
Gabriela Matela1, Peng Gao2, Gernot Guigas1
1Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Chemical Communications (Cambridge, England)
|January 4, 2017
Abstract:
Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.
Related Concept Videos
01:37Super-resolution Fluorescence Microscopy
14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
02:11Reporter Genes
13.6K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.6K