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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Neuron-specific splicing.

Nor Hakimah Ab Hakim1, Burhanuddin Yeop Majlis, Hitoshi Suzuki

  • 1Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia.

Bioscience Trends
|January 5, 2017
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Summary
This summary is machine-generated.

Alternative splicing, a key gene expression regulator, involves complex protein control. This review highlights neuronal splicing regulators and their links to diseases like Alzheimer's and Autism.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience

Background:

  • Alternative splicing is a crucial gene expression regulatory mechanism in eukaryotes, affecting over 90% of human genes.
  • Common alternative splicing events include exon skipping, alternative splice sites, intron retention, and mutually exclusive exons.
  • Splicing events are precisely controlled by regulatory proteins mediating positive and negative regulation.

Purpose of the Study:

  • To review neuronal splicing regulators and their functions.
  • To examine the role of the RBFox protein family in splicing regulation.
  • To explore the connection between splicing factors and neuronal diseases such as Alzheimer's disease (AD) and Autism Spectrum Disorder (ASD).

Main Methods:

  • Literature review focusing on neuronal splicing regulation.
  • In-depth discussion of notable neuronal splicing regulators.
  • Case study analysis of RBFox protein family-mediated splicing regulation.

Main Results:

  • Identification and detailed discussion of key neuronal splicing regulators.
  • Exemplification of splicing regulation by the RBFox protein family.
  • Association of splicing factors with the pathogenesis of AD and ASD.

Conclusions:

  • Neuronal splicing regulators play critical roles in nervous system function.
  • Dysregulation of splicing factors is implicated in neurodevelopmental and neurodegenerative diseases.
  • Further research is needed to elucidate the precise mechanisms linking splicing factors to neuronal disease.