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Related Experiment Video

Updated: Mar 9, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods.

F Philip¹, E E Ha¹, M A Seeliger¹

¹Center for Developmental Genetics, Stony Brook University School of Medicine, Stony Brook, NY, United States.

Methods in Enzymology

|January 9, 2017

Summary

This summary is machine-generated.

Phospholipase D (PLD) enzymes are crucial for cell signaling, immune responses, and cancer. This study focuses on human PLD6, detailing its production, purification, and RNA-degrading activity, vital for fertility and neuronal health.

Keywords:

Biochemical assay Endonuclease Fluorescent sensor Phosphatidic acid Phospholipase D

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Last Updated: Mar 9, 2026

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Defining Substrate Specificities for Lipase and Phospholipase Candidates

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

The phospholipase D (PLD) superfamily regulates critical cellular processes like immune responses, cancer, and spermatogenesis.
PLD enzymes generate phosphatidic acid (PA), a key signaling lipid, and have roles in RNA interference.

Purpose of the Study:

To detail the production, purification, and in vitro endonuclease activity of human PLD6.
To explore the functions of mitochondrial-tethered PLD6 in fertility, cancer, and neuronal homeostasis.

Main Methods:

Production and purification of human PLD6.
In vitro analysis of PLD6's endonuclease activity on RNA transcripts.
Brief review of PA detection and imaging methods.

Main Results:

Successful production and purification of human PLD6.
Demonstration of in vitro endonuclease activity of PLD6.
Established methods for analyzing PLD6 activity.

Conclusions:

Human PLD6 is an RNA-degrading enzyme with significant roles in fertility, cancer, and neuronal function.
Further research into PLD6 mechanisms can inform therapeutic strategies for related diseases.