Research on a New Micro-Volume Fluorescence Capillary Biosensor Assay for Sequentially Quantifying Pyruvate and...

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Biotechnology

Background:

• Pyruvate (PA) and lactate (LA) are key biomarkers in clinical diagnostics.
• Accurate and efficient quantification of PA and LA is crucial for disease monitoring.
• Existing methods may require large sample volumes, pre-separation, or lack reusability.

Purpose of the Study:

• To develop and optimize a micro-volume fluorescence capillary biosensor for sequential determination of PA and LA.
• To evaluate the biosensor's performance in human serum samples.
• To highlight the advantages of the developed biosensor for clinical applications.

Main Methods:

• Optimization of micro-volume fluorescence capillary biosensor conditions.
• Immobilization of lactate dehydrogenase (LDH) on a capillary for enzyme reuse.
• Sequential quantification of LA and PA using fluorospectrophotometry.
• Validation using human serum samples.

Main Results:

• Optimized biosensor achieved sequential quantification of LA (0.10–1.2 mM) and PA (4–120 μM).
• High recovery rates (97–106%) for PA and LA in human serum.
• Low detection limits: 0.023 mM for LA and 0.87 μM for PA, with good reproducibility (RSD < 1.89%).

Conclusions:

• The developed fluorescence capillary biosensor is accurate, reliable, and cost-effective.
• Enzyme immobilization allows for reusability, reducing reagent consumption.
• The biosensor shows significant potential for clinical and biological assays of PA and LA.