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Versatile tissue lasers based on high-Q Fabry-Pérot microcavities
Yu-Cheng Chen1, Qiushu Chen1, Tingting Zhang2
1Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA. xsfan@umich.edu.
Lab on a Chip
|January 19, 2017
Summary
Researchers developed a versatile tissue laser platform using fluorophore-stained tissues in a microcavity. This biophotonics tool achieves distinct lasing emissions for multiplexed biochemical detection in complex biological samples.
Area of Science:
- Biophotonics
- Biomedical Engineering
- Materials Science
Background:
- Biolasers offer advanced biochemical detection and clinical applications.
- Lasing from biomolecules and single cells has been achieved.
- Tissues represent a more complex and practically significant biological environment.
Purpose of the Study:
- To develop a versatile tissue laser platform for next-generation diagnostics.
- To investigate lasing properties in various tissue types and staining conditions.
- To enable highly multiplexed detection in biological samples.
Main Methods:
- Fabrication of a high-Q Fabry-Pérot microcavity housing fluorophore-stained tissues.
- Utilizing fluorescein isothiocyanate (FITC) and boron-dipyrromethene (BODIPY) for staining muscle and adipose tissues.
- Investigating the effects of tissue structure, thickness, and dye concentration on lasing.
Main Results:
- Achieved distinct lasing emissions from muscle and adipose tissues with a low threshold (∼10 μJ mm-2).
- Demonstrated successful lasing from FITC conjugates targeting F-actin in muscle tissue.
- Showcased clear distinction and control of lasing emissions from FITC and BODIPY despite spectral overlap, enabling multiplexed detection.
Conclusions:
- The developed tissue laser platform is highly versatile and applicable to various tissues and diseases.
- This platform provides a novel tool for biological and biomedical applications, including tissue diagnostics and monitoring.
- Enables precise, multiplexed detection crucial for tissue engineering and disease screening.
