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Overview of Cell Signaling

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Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
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Updated: Jun 27, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Published on: October 15, 2013

Microsphere cytometry to interrogate microenvironment-dependent cell signaling.

Henriette Christie Ertsås¹, Garry P Nolan², Mark A LaBarge³

¹Department of Biomedicine, Center for Cancer Biomarkers, University of Bergen, Bergen, Norway. Jim.Lorens@uib.no.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|January 20, 2017

Summary

This summary is machine-generated.

Researchers developed a novel microsphere flow cytometry method to measure single-cell signaling. This technique reveals unique cell signatures by mimicking microenvironments, distinguishing normal from neoplastic cells.

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Area of Science:

Cellular biology
Biotechnology
Immunology

Background:

Cellular signaling is governed by microenvironmental cues, influencing both normal and diseased states.
Understanding these signals is crucial for diagnostics and therapeutics.

Purpose of the Study:

To develop a novel method for measuring single-cell signaling responses.
To investigate how microenvironmental cues impact cellular signaling networks.
To differentiate between normal and neoplastic cells based on their signaling signatures.

Main Methods:

A microsphere-based flow cytometry approach was developed.
Single cells were adhered to microspheres displaying mimetic microenvironments (extracellular matrix proteins, soluble factors).
Temporal signaling responses were measured using multiparametric flow cytometry and fluorophore-conjugated antibodies.

Main Results:

The novel method successfully measured temporal signaling responses in single adherent cells.
Mimetic microenvironments generated distinct cellular signal networks.
Unique cell signatures were identified in normal and patient-derived neoplastic cells.

Conclusions:

The microsphere-based flow cytometry approach is effective for analyzing single-cell signaling.
Microenvironment-mimetic combinations reveal distinct signaling networks and cell-specific signatures.
This technology holds potential for distinguishing cell states and informing disease understanding.