Related Experiment Video
Updated: Mar 8, 2026

08:49
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Published on: March 14, 2021
4.7K
Fluorogenic probes to monitor cytosolic phospholipase A2 activity
Cheng Yang Ng1, Timothy Xiong Wei Kwok1, Francis Chee Kuan Tan2
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. chmlamyl@nus.edu.sg.
Abstract:
Arachidonic acid derivatives equipped with either one or two fluorescent groups attached to the tip of the alkyl chains were synthesized and shown to function as inhibitor and substrate probes of cPLA2. The inhibitor probe was demonstrated to perform dual functions of inhibition and imaging while the substrate probe could be used for activity assay.

