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Related Experiment Videos

Molecular mapping within the mouse albino-deletion complex.

D K Johnson1, R E Hand, E M Rinchik

  • 1Biology Division, Oak Ridge National Laboratory, TN.

Proceedings of the National Academy of Sciences of the United States of America
|November 1, 1989
PubMed
Summary

Researchers developed a method to map mouse genome regions using deletion mutations. This technique identified a new DNA probe, D7OR1, crucial for understanding embryonic development and genetic disorders.

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Area of Science:

  • Genetics
  • Genomics
  • Developmental Biology

Background:

  • Induced germ-line deletion mutations in mice offer a flexible system for studying large mammalian genome segments.
  • The albino-deletion complex on mouse chromosome 7 is a key region for investigating developmental phenotypes.

Purpose of the Study:

  • To generate molecular probes for the mouse chromosome 7 region associated with the albino-deletion complex.
  • To rapidly map anonymous DNA probes to chromosomal segments linked to germ-line deletion mutations.

Main Methods:

  • Screening random anonymous DNA clones against Mus musculus-Mus spretus F1 hybrid DNAs carrying albino deletions.
  • Utilizing restriction fragment length polymorphisms to identify clones within deletion intervals.
  • Submapping using a panel of 27 smaller deletions and standard three-point linkage analysis.

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Main Results:

  • One informative clone, defining the D7OR1 locus, was mapped within two large deletions (6-11 centimorgans).
  • D7OR1 was localized distal to a critical preimplantation embryo survival region and proximal to the globin beta-chain (Hbb) locus.
  • The mapping results were confirmed by three-point linkage analysis.

Conclusions:

  • The strategy effectively selects and rapidly maps anonymous DNA probes to deletion mutation regions.
  • This approach aids in generating detailed physical and functional maps of genomic regions linked to developmental mutations.
  • The D7OR1 locus's position provides insights into genetic regulation of early embryonic development.