Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased
Xudong Zhu1, Bessembayev Arman1, Ju Chu2
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Objectives:
To develop an efficient cost-effective screening process to improve production of glucoamylase in Aspergillus niger.
Results:
The cultivation of A. niger was achieved with well-dispersed morphology in 48-deep-well microtiter plates, which increased the throughput of the samples compared to traditional flask cultivation. There was a close negative correlation between glucoamylase and its pH of the fermentation broth. A novel high-throughput analysis method using Methyl Orange was developed. When compared to the conventional analysis method using 4-nitrophenyl α-D-glucopyranoside as substrate, a correlation coefficient of 0.96 by statistical analysis was obtained.
Conclusion:
Using this novel screening method, we acquired a strain with an activity of 2.2 × 103 U ml-1, a 70% higher yield of glucoamylase than its parent strain.
