Related Experiment Video
Updated: Mar 8, 2026
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Published on: August 17, 2022
Optimal euploid embryo transfer strategy, fresh versus frozen, after preimplantation genetic screening with next
Alison Coates1, Allen Kung2, Emily Mounts3
1Oregon Reproductive Medicine, Portland, Oregon; School of Biosciences, University of Kent, Canterbury, United Kingdom.
Objective:
To compare two commonly used protocols (fresh vs. vitrified) used to transfer euploid blastocysts after IVF with preimplantation genetic screening.
Design:
Randomized controlled trial.
Setting:
Private assisted reproduction center.
Patient(S):
A total of 179 patients undergoing IVF treatment using preimplantation genetic screening.
Intervention(S):
Patients were randomized at the time of hCG administration to either a freeze-all cycle or a fresh day 6 ET during the stimulated cycle.
Main Outcome Measure(S):
Implantation rates (sac/embryo transferred), ongoing pregnancy rates (PRs) (beyond 8 weeks), and live birth rate per ET in the primary transfer cycle.
Result(S):
Implantation rate per embryo transferred showed an improvement in the frozen group compared with the fresh group, but not significantly (75% vs. 67%). The ongoing PR (80% vs. 61%) and live birth rates (77% vs. 59%) were significantly higher in the frozen group compared with the fresh group.
Conclusion(S):
Either treatment protocol investigated in the present study can be a reasonable option for patients. Freezing all embryos allows for inclusion of all blastocysts in the cohort of embryos available for transfer, which also results in a higher proportion of patients reaching ET. These findings suggest a trend toward favoring the freeze-all option as a preferred transfer strategy when using known euploid embryos.
Clinical Trial Registration Number:
NCT02000349.
