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Related Experiment Video

Updated: Mar 8, 2026

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
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Diffuse Optical Spectroscopy and Imaging to Detect and Quantify Adipose Tissue Browning.

U S Dinish1, Chi Lok Wong1, Sandhya Sriram2

  • 1Bio Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), Singapore.

Scientific Reports
|February 2, 2017
PubMed
Summary

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Optical imaging techniques like diffuse reflectance spectroscopy and multispectral imaging can now rapidly detect and quantify the browning of white adipose tissue (WAT) in mice, offering a faster alternative to traditional methods.

Area of Science:

  • Metabolic research
  • Adipose tissue biology
  • Optical imaging techniques

Background:

  • Brown adipose tissue (BAT) is metabolically beneficial due to its calorie-burning heat production.
  • Converting white adipose tissue (WAT) to brown-like 'beige' adipocytes is a key research area.
  • Conventional methods for analyzing WAT browning are time-consuming and lack accurate quantification.

Purpose of the Study:

  • To introduce diffuse reflectance spectroscopy (DRS) and multispectral imaging (MSI) as novel tools for adipose tissue analysis.
  • To detect and quantify the browning process in white adipose tissue (WAT) in vivo.
  • To establish a faster, more accurate method for assessing WAT browning compared to traditional assays.

Main Methods:

  • Application of diffuse reflectance spectroscopy (DRS) and multispectral imaging (MSI) to monitor WAT browning in mice.

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  • Induction of WAT browning using beta-adrenergic agonist injections.
  • Validation of optical techniques against standard molecular (UCP1, PGC-1α gene/protein expression) and histological analyses.

    • Main Results:

    • Demonstrated successful detection and time-dependent quantification of WAT browning using DRS and MSI.
    • Optical measurements correlated well with established molecular and histological markers of browning.
    • Confirmed the ability of optical methods to track the increase in beige/brown adipocyte characteristics.

    Conclusions:

    • Diffuse reflectance spectroscopy (DRS) and multispectral imaging (MSI) provide a rapid and effective means to detect and quantify WAT browning.
    • These optical imaging approaches offer a significant advancement over conventional, slower methods.
    • The developed imaging techniques hold potential for cost-effective, real-time quantification of adipose tissue browning.