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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.0K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Raman Spectroscopy: Overview01:20

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Related Experiment Video

Updated: Jan 13, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

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Noninvasive assessment of skin barrier function: evaluating ceramide-based moisturizer using confocal Raman

Poongkulali Rajarahm1, Renzhe Bi1, Amalina Ebrahim Attia1

  • 1Agency for Science Technology and Research, Translational Biophotonics Laboratory, A*STAR Skin Research Labs, Singapore.

Journal of Biomedical Optics
|January 8, 2026
PubMed
Summary

Confocal Raman spectroscopy (CRS) offers sensitive skin hydration and ceramide level analysis. A ceramide-based cream significantly improved these parameters in eczema patients, outperforming a standard moisturizer.

Keywords:
atopic dermatitisceramideconfocal Raman spectroscopydermatologyhandheld probe

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Area of Science:

  • Dermatology and Biophysics
  • Non-invasive Skin Analysis

Background:

  • Atopic dermatitis and hand eczema involve impaired skin barrier function.
  • Effective monitoring tools are crucial for assessing treatment efficacy in these conditions.

Purpose of the Study:

  • To quantitatively assess skin physiological and biochemical changes after applying a ceramide-based moisturizer versus a control.
  • To compare the efficacy of a test cream against a control cream in healthy volunteers and eczema patients.

Main Methods:

  • Utilized Confocal Raman spectroscopy (CRS) and skin physiological assessments.
  • Evaluated water content and ceramide levels in superficial and deeper skin layers.
  • Compared a ceramide-based test cream with an aqueous control cream.

Main Results:

  • CRS detected significant increases in skin water content with the test cream, indicating enhanced hydration.
  • The test cream significantly boosted ceramide levels in both superficial and deeper skin layers.
  • The control cream only increased ceramide levels in the superficial stratum corneum.

Conclusions:

  • The ceramide-based test cream demonstrates a significant positive impact on hand eczema.
  • The test cream shows potential for improving both skin barrier function and biochemical properties in eczema.
  • Confocal Raman spectroscopy (CRS) is a highly sensitive method for evaluating moisturizer efficacy.