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Endoscopic micro-optical coherence tomography with extended depth of focus using a binary phase spatial filter.

Junyoung Kim, Jingchao Xing, Hyeong Soo Nam

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    This study introduces an endoscopic micro-optical coherence tomography (μOCT) probe that extends imaging depth of focus. This innovation enables higher resolution 3D microstructure imaging for potential clinical applications.

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    Area of Science:

    • Biomedical optics
    • Medical imaging technology
    • Optical engineering

    Background:

    • Micro-optical coherence tomography (μOCT) offers high-resolution 3D imaging of biological samples.
    • High numerical aperture (NA) lenses in μOCT result in a limited depth of focus (DOF), hindering deep tissue imaging.
    • Developing endoscopic μOCT probes is crucial for clinical translation but faces optical and spatial challenges.

    Purpose of the Study:

    • To develop an endoscopic μOCT probe with an extended DOF.
    • To improve the imaging depth capabilities of μOCT for biological samples.
    • To assess the feasibility of the developed probe for clinical applications.

    Main Methods:

    • Fabrication of an endoscopic μOCT probe incorporating a binary phase spatial filter.
    • Utilizing a broadband light source and high NA lens for μOCT imaging.
    • Characterization of axial and lateral resolution and DOF extension using latex beads.

    Main Results:

    • The developed μOCT probe achieved an axial resolution of 2.49 μm and a lateral resolution of 2.59 μm.
    • The probe demonstrated a DOF extended by a factor of 2 compared to conventional μOCT systems.
    • Successful ex vivo imaging of a rabbit iliac artery confirmed the probe's clinical feasibility.

    Conclusions:

    • The novel binary phase spatial filter effectively extends the DOF in endoscopic μOCT.
    • The developed endoscopic μOCT probe provides high-resolution 3D imaging with enhanced depth penetration.
    • This technology shows significant potential for in vivo clinical applications in vascular imaging and beyond.