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Published on: June 6, 2017
C-Myc regulates radiation-induced G2/M cell cycle arrest and cell death in human cervical cancer cells
Fengmei Cui1,2, Jun Hou3, Chengcheng Huang1,2
1Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
Aim:
The study was conducted to investigate the role of c-Myc in the regulation of ionizing radiation-induced cell cycle arrest and cell death in human cervical cancer cells.
Methods:
Control and c-Myc-silenced Hela cells were collected at different time points after 60 Co γ-ray radiation. Flow cytometry was used to measure cell cycle distribution and apoptosis. Immunofluorescence was applied to determine the percentage of cells in M phase. Transmission electron microscopy and immunoblotting were used to detect the induction of autophagy after radiation. Immunoblotting was also used to measure the expression levels of apoptosis-related proteins.
Results:
In c-Myc-silenced cells, radiation induced delayed but long-lasting G2/M arrest and an abnormal M phase compared with the control. In addition, c-Myc knockdown significantly inhibited apoptotic cell death induced by radiation. Meanwhile, radiation-induced autophagy appeared stronger in c-Myc-silenced cells. Mechanically, we found that Caspase 8 and survivin expression was decreased in c-Myc-silenced Hela-630 cells.
Conclusions:
These data showed that c-Myc serves as a co-regulator in radiation-induced G2/M cell cycle arrest and cell death in human cervical cancer cells.
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