A selective chemosensor for fluoride ion and its interaction with Calf Thymus DNA

Area of Science:

• Chemical Synthesis
• Analytical Chemistry
• Biophysical Chemistry

Background:

• Schiff bases are versatile organic compounds with diverse applications.
• Fluoride ion detection is crucial in environmental and health monitoring.
• Understanding interactions between small molecules and DNA is vital for drug development.

Purpose of the Study:

• Synthesize and characterize a novel amido-Schiff base.
• Evaluate its potential as a selective fluoride ion sensor.
• Investigate its binding interactions with Calf Thymus-DNA (CT-DNA).

Main Methods:

• Condensation reaction for Schiff base synthesis.
• Spectroscopic methods (UV-vis) and visual color change for fluoride sensing.
• Molecular modeling, UV-vis spectroscopy, circular dichroism, and thermal melting studies for DNA binding analysis.

Main Results:

• Successfully synthesized amido-Schiff base 1 (N 1 , N 3 -bis (2-nitrobenzylidene)benzene-1,3-dicabohydrazide).
• Compound 1 demonstrated high selectivity and sensitivity for fluoride ion detection, with a visual color change.
• Effective binding with CT-DNA was confirmed through experimental and theoretical studies, with a binding energy of -7.55 to -7.50 kcal/mol.

Conclusions:

• The synthesized amido-Schiff base is a promising visual sensor for fluoride ions, applicable even in toothpaste.
• The compound exhibits significant interaction with CT-DNA, suggesting potential applications in biophysical studies.
• This dual functionality highlights the versatility of the designed amido-Schiff base.