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Updated: Aug 9, 2026

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
Published on: August 26, 2016
[The fatty acid composition of Haemophilus and Pasteurella multocida cells as a phylogenetic marker]
Abstract:
When grown on meat-peptone agar with heated blood, different Haemophilus species (H. influenzae, H. parahaemolyticus, H. parasuis, H. pleuropneumoniae), including different H. influenzae serovars (a, b, c, d, e, f), and Pasteurella multocida have identical fatty acid composition, characterized by the prevalence of fatty acids with 16 carbon atoms, constituting about 70% and more of the total number of fatty acids, and a low level of fatty acids with 18 carbon atoms. P. multocida strains cultivated on meat-peptone agar with unheated blood have a greatly increased content of fatty acids with 18 carbon atoms, while the content of fatty acids with 16 carbon atoms is much lower. The identity of fatty acid composition under similar cultivation conditions, together with their similarity in other phenetic signs, is indicative of close phylogenic relationship between bacteria belonging to the genus Haemophilus and P. multocida.
Insights
Fatty acid analysis reveals that Haemophilus species and Pasteurella multocida share similar compositions when grown on heated blood media. This suggests a close phylogenetic relationship between these bacterial genera.
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Biochemistry
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