Related Experiment Video
Updated: Mar 8, 2026
11:46
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU
Published on: August 22, 2019
11.7K
ACTH Action on Messenger RNA Stability Mechanisms.
Agnès Desroches-Castan1, Jean-Jacques Feige1, Nadia Cherradi1
1Institut National de la Santé et de la Recherche Médicale, INSERM U1036, Grenoble, France; Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut de Biosciences et Biotechnologies de Grenoble, Laboratoire Biologie du Cancer et de l'Infection, Grenoble, France; Université Grenoble Alpes, Unité Mixte de Recherche-S1036, Grenoble, France.
Frontiers in Endocrinology
|February 7, 2017
Summary
Messenger RNA (mRNA) stability controls gene expression, especially during hormonal changes. RNA-binding proteins regulate mRNA decay, and their dysfunction is linked to diseases.
Area of Science:
- Molecular Biology
- Endocrinology
- Gene Expression Regulation
Background:
- mRNA stability is a key regulator of dynamic gene expression, responding to environmental cues like hormones.
- RNA-binding proteins (RNA-BP) interacting with 3'-untranslated region motifs, such as AU-rich elements (AREs), modulate mRNA stability.
- Dysregulation of RNA-BP is implicated in various human diseases, highlighting the importance of mRNA stability control.
Purpose of the Study:
- To investigate the role and regulation of mRNA stability mechanisms in the adrenal cortex.
- To explore the significance of RNA-binding proteins in endocrine function.
Main Methods:
- Analysis of mRNA stability regulatory proteins in the adrenal cortex.
- Investigating the impact of hormonal stimulation (ACTH/cAMP) on RNA-BP expression and localization.
Main Results:
- ACTH/cAMP signaling affects the expression/phosphorylation of the mRNA-destabilizing protein TIS11b/BRF1.
- Hormonal stimulation influences the subcellular localization of the mRNA-stabilizing protein Human antigen R.
- These findings suggest mRNA stability is a relevant regulatory layer in endocrinology.
Conclusions:
- mRNA stability control by RNA-binding proteins is crucial for rapid gene expression adjustments in response to hormonal signals.
- The adrenal cortex utilizes mRNA stability mechanisms, involving proteins like TIS11b/BRF1 and Human antigen R, for endocrine regulation.
- Further research into RNA-BP in endocrinology is warranted to understand their role in health and disease.
Related Concept Videos
01:53RNA Stability
36.0K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
36.0K
02:51mRNA Stability and Gene Expression
6.8K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.8K
02:51mRNA Stability and Gene Expression
3.6K
3.6K
02:41Chromatin Structure Regulates pre-mRNA Processing
8.4K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
8.4K
01:23Regulation of Expression at Multiple Steps
1.5K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
01:20Types of RNA
10.2K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
10.2K