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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
Published on: February 28, 2017
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Phenotype and multipotency of rabbit (Oryctolagus cuniculus) amniotic stem cells
Jéssica Borghesi1,2, Lara Carolina Mario3, Ana Claudia Oliveira Carreira4
1Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, SP, Brazil. jehborghesi@hotmail.com.
Stem Cell Research & Therapy
|February 9, 2017
Summary
Rabbit amniotic membrane yields multipotent mesenchymal stem cells. These cells show promise for future cell therapy applications due to their proliferative capacity and differentiation potential.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Tissue Engineering
Background:
- Embryonic stem cells face ethical and religious challenges, while adult stem cells have limited plasticity.
- Foetal stem cells from extra-embryonic tissues offer an alternative, exhibiting high proliferation, in vitro growth, and unique immunogenic properties.
- The amniotic membrane is emerging as a significant source for novel stem cell isolation.
Purpose of the Study:
- To culture and characterize mesenchymal progenitor cells from rabbit amniotic membrane.
- To investigate the differentiation potential of these amniotic membrane-derived cells.
- To assess the safety and therapeutic applicability of these stem cells.
Main Methods:
- Mesenchymal progenitor cells were isolated from rabbit foetal amniotic membranes using the explant technique.
- Cells were cultured in DMEM-HIGH glucose medium under standard cell culture conditions (37°C, 5% CO2).
- Characterization involved assessing morphology, proliferation, stem cell markers, differentiation capacity, and in vivo tumor formation.
Main Results:
- Cultured cells exhibited fibroblast-like morphology, high proliferative capacity, and adherence to culture plates.
- Positive expression of cytoskeleton, mesenchymal stem cell, proliferation, and pluripotency markers was observed; CD45 (hematopoietic marker) was negative.
- Cells demonstrated successful differentiation into osteogenic, adipogenic, and chondrogenic lineages, with no tumor formation upon injection into nude mice.
Conclusions:
- Multipotent mesenchymal stem cells can be successfully obtained from the rabbit amniotic membrane.
- These cells possess desirable characteristics for potential use in future cell therapy strategies.
- Rabbit amniotic membrane-derived stem cells represent a promising, safe, and versatile cell source for regenerative medicine.
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