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Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
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Polymeric nanoparticles containing diazepam: preparation, optimization, characterization, in-vitro drug release and
Sarvesh Bohrey1, Vibha Chourasiya1, Archna Pandey1
1Department of Chemistry, Dr. Harisingh Gour University, Sagar, Madhya Pradesh 470003 India.
Nano Convergence
|February 14, 2017
Summary
Biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles were formulated to deliver diazepam, an anticonvulsant drug. The study optimized preparation variables to achieve spherical nanoparticles with sustained drug release.
Area of Science:
- Nanotechnology
- Materials Science
- Pharmaceutical Sciences
Background:
- Biodegradable nanoparticles, particularly poly(lactic-co-glycolic acid) (PLGA), are crucial for advanced drug delivery systems.
- PLGA nanoparticles offer biocompatibility and controlled drug release, making them ideal for therapeutic applications.
- Diazepam, a widely used anticonvulsant, requires effective delivery systems for conditions like epilepsy and anxiety.
Purpose of the Study:
- To formulate diazepam-loaded PLGA nanoparticles using the emulsion solvent evaporation technique.
- To investigate the impact of preparation variables on nanoparticle size and morphology.
- To characterize the formulated nanoparticles and evaluate their in vitro drug release kinetics.
Main Methods:
- Emulsion solvent evaporation technique was employed for nanoparticle formulation.
- Polyvinyl alcohol (PVA) served as the stabilizing agent.
- Characterization involved photon correlation spectroscopy (PCS), transmission electron microscopy (TEM), and zeta potential measurements.
Main Results:
- Preparation variables such as sonication time, polymer content, and surfactant concentration significantly influenced nanoparticle size.
- Spherical diazepam-loaded PLGA nanoparticles were successfully produced, with sizes under 250 nm and a zeta potential of -23.3 mV.
- In vitro drug release studies demonstrated sustained diazepam release, consistent with the Korsmeyer-Peppas kinetic model.
Conclusions:
- The emulsion solvent evaporation method is effective for producing diazepam-loaded PLGA nanoparticles.
- Optimized preparation parameters yield nanoparticles with desirable size, morphology, and surface charge for drug delivery.
- The sustained release profile suggests potential for improved therapeutic efficacy and reduced dosing frequency.
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