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In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
Published on: August 9, 2017
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Cardiosphere-derived cells do not improve cardiac function in rats with cardiac failure
Taís Hanae Kasai-Brunswick1,2,3, Andréa Rodrigues da Costa2, Raiana Andrade Quintanilha Barbosa1,2
1Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, n°373, room G2-053, CEP:21941-902, Rio de Janeiro, RJ, Brazil.
Stem Cell Research & Therapy
|February 17, 2017
Summary
Cardiosphere-derived cells (CDC) did not improve cardiac function or remodeling in a rat model of heart failure 60 days after myocardial infarction. This study challenges the effectiveness of CDC therapy for post-ischemic heart failure.
Area of Science:
- Cardiology
- Regenerative Medicine
- Stem Cell Therapy
Background:
- Heart failure is a growing global health concern with significant costs.
- Endogenous cardiac stem cells are hypothesized to exist in adult hearts, with cardiosphere-derived cells (CDC) being a potential candidate.
- Previous studies suggested CDC therapy improves cardiac function after myocardial infarction (MI) in rodents.
Purpose of the Study:
- To evaluate the therapeutic potential of cardiosphere-derived cells (CDC) in a rat model of heart failure, 60 days after myocardial infarction (MI).
Main Methods:
- Cardiosphere-derived cells (CDC) were isolated from human and rat hearts.
- Wistar rats underwent MI and were treated 60 days later with human CDC (hCDC) or rat CDC (rCDC), or placebo.
- Cell tracking, echocardiography, and Langendorff analysis were used to assess cardiac function and remodeling.
Main Results:
- CDC exhibited a predominantly mesenchymal phenotype.
- Bioluminescence tracking showed over 85% signal decrease within 5-7 days post-therapy.
- No significant differences in ejection fraction, cardiac volumes, or infarct size were observed between CDC-treated groups and placebo.
Conclusions:
- This study challenges the efficacy of cardiosphere-derived cells (CDC) for treating post-ischemic heart failure.
- CDC therapy did not demonstrate significant improvements in cardiac function or remodeling in this chronic MI rat model.
Keywords:
BioluminescenceCardiosphere-derived cellsCell therapyHeart failureMyocardial infarctionStem cellsMore Related Videos
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