Expression of MicroRNA in Host Cells Infected with Helicobacter pylori
Jun-Won Chung1, Seok Hoo Jeong2, Sun Mi Lee3
1Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Korea.
Background/Aims:
MicroRNAs (miRNAs) regulate gene expression. We assess miRNA regulation by Helicobacter pylori infection and elucidate their role in H. pylori-infected gastric epithelial cells.
Methods:
The relationship between miRNA expression and DNA methylation was examined. Cells were treated with the nuclear factor-kappaB (NF-κB) inhibitor Bay 11-7082 to determine the relationship between miRNA expression and NF-κB signal transduction.
Results:
In the negative control cells infected with H. pylori 26695, the expression of six miRNAs was increased, whereas the expression of five miRNAs was decreased. The expression of upregulated miRNAs was increased when the host cells were treated with H. pylori and an NF-κB inhibitor. miR-127-5p, -155, and -181 were associated with increased interleukin 6 (IL-6) secretion in H. pylori infected cells treated with anti-miRNA. The expression of miR-155, -127-5p, -195, -216, -206, and -488 increased by approximately 3-fold following treatment with the methylation inhibitor Aza.
Conclusions:
We found novel miRNAs in H. pylori-infected negative control cells using miRNA microarrays. Upregulated miRNA expression was inversely related to the transcription of NF-κB. miR-195 and miR-488 appear to play a pivotal role in controlling IL-6 activity in H. pylori infection. miRNA expression in H. pylori infection was affected by methylation.
Insights
Helicobacter pylori infection alters microRNA (miRNA) expression in gastric cells. Specific miRNAs, like miR-195 and miR-488, play key roles in regulating interleukin-6 (IL-6) secretion and are influenced by DNA methylation and NF-κB signaling.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- MicroRNAs (miRNAs) are crucial regulators of gene expression.
- Helicobacter pylori infection is a significant factor in gastric diseases.
- Understanding miRNA dysregulation in H. pylori infection is vital for therapeutic strategies.
Purpose of the Study:
- To investigate the impact of H. pylori infection on miRNA expression in gastric epithelial cells.
- To elucidate the role of specific miRNAs in H. pylori-induced cellular responses.
- To explore the relationship between miRNA expression, DNA methylation, and NF-κB signaling.
Main Methods:
- miRNA expression profiling in H. pylori-infected cells.
- Treatment with NF-κB inhibitor (Bay 11-7082) and methylation inhibitor (Aza).
- Assessment of interleukin-6 (IL-6) secretion using anti-miRNA treatments.
Main Results:
- H. pylori infection altered the expression of multiple miRNAs.
- Upregulated miRNA expression showed an inverse correlation with NF-κB activity.
- miR-195 and miR-488 were identified as key regulators of IL-6 secretion in infected cells.
- DNA methylation significantly influenced miRNA expression patterns.
Conclusions:
- Novel miRNAs were identified in H. pylori-infected gastric cells.
- NF-κB signaling and DNA methylation are critical modulators of miRNA expression during H. pylori infection.
- Specific miRNAs, including miR-195 and miR-488, are pivotal in controlling IL-6 activity, offering potential therapeutic targets.
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