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Single-step procedure for the extraction and purification of leukotrienes B4, C4 and D4
R Richmond1, N C Turner, N Maltby
1Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
Journal of Chromatography
|July 3, 1987
Abstract:
A rapid and simple method for the on-line concentration and high-performance liquid chromatographic (HPLC) purification of the leukotrienes in good yield from biological fluids is described. Readily available antisera are used in conjunction with this system to give a specific and sensitive assay for leukotrienes B4, C4 and D4 with sub-nanogram limits of detection. Tritium-labelled leukotrienes are used as internal standards, both to locate the leukotrienes post-HPLC and to accurately determine recoveries.