Dentine matrix proteins: isolation and effects on human pulp cells.
M Widbiller1, A Eidt1, S R Lindner1
1Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.
International Endodontic Journal
|February 18, 2017
Summary
A new method efficiently isolates dentine matrix proteins (eDMP). These proteins promote dental pulp cell migration, differentiation, and mineralization, suggesting potential for dental pulp regeneration and engineering.
Area of Science:
- Biomaterials Science
- Cell Biology
- Regenerative Medicine
Background:
- Dentine matrix proteins play crucial roles in pulp biology.
- Efficient isolation of these proteins is essential for regenerative applications.
- Understanding eDMP effects on pulp cells informs tissue engineering strategies.
Purpose of the Study:
- To develop a simplified protocol for isolating and concentrating human dentine matrix proteins (eDMP).
- To investigate the impact of eDMP on human dental pulp cell behavior, including viability, proliferation, migration, differentiation, and mineralization.
Main Methods:
- Dentine matrix proteins were isolated, purified, and concentrated.
- Human pulp cells were cultured with varying eDMP concentrations.
- Cell viability, proliferation, apoptosis, chemotaxis, gene expression (odontoblastic markers), and mineralization were assessed using DNA, MTT, flow cytometry, Boyden chamber, qPCR, and alizarin red staining.
Main Results:
- High eDMP concentrations showed antiproliferative effects but did not impact cell viability or apoptosis.
- eDMP demonstrated a concentration-dependent chemotactic effect on pulp cells.
- eDMP induced odontoblast-like cell differentiation and mineralization.
Conclusions:
- A novel, effective, and time-saving protocol for eDMP isolation was established.
- eDMP promotes pulp cell chemotaxis, differentiation, and mineralization.
- Endogenous dentine matrix proteins hold promise for dental pulp regeneration and engineering strategies.
