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Updated: Mar 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Advances in Clinical Mass Spectrometry.
1University of California San Francisco, San Francisco, CA, United States.
Mass spectrometry (MS) offers superior sensitivity and specificity compared to immunoassays for clinical diagnostics. This review explores MS applications in therapeutic drug monitoring, toxicology, and steroid hormone analysis, highlighting technological advancements.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Biomedical Science
Background:
- Immunoassay technology automated clinical laboratories but has limitations in sensitivity and specificity.
- Certain analytes and patient populations require more precise measurement methods.
- Clinical laboratories are re-evaluating mass spectrometry for enhanced diagnostic capabilities.
Purpose of the Study:
- To review the clinical applications of mass spectrometry (MS).
- To discuss advancements in MS technology and emerging clinical uses.
- To highlight MS's role in therapeutic drug monitoring, toxicology, and steroid hormone analysis.
Main Methods:
- Review of existing literature and clinical case studies.
- Analysis of technological progress in mass spectrometry instrumentation.
- Evaluation of mass spectrometry's performance in specific clinical assays.
Main Results:
- Mass spectrometry provides enhanced sensitivity and specificity over immunoassays.
- MS enables the development of novel assays for previously unmeasurable analytes.
- Established clinical applications include therapeutic drug monitoring, toxicology, and steroid hormone analysis.
Conclusions:
- Mass spectrometry is a valuable tool for overcoming immunoassay limitations in clinical diagnostics.
- Technological advancements continue to expand the utility of MS in healthcare.
- MS plays a critical role in specialized areas of clinical laboratory testing.
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