In-vivo analysis of morphogenesis in plants

Although many molecular regulators of morphogenesis have been identified in plants, it remains largely unknown how the molecular networks influence local cell shape and how cell growth, form, and position are coordinated during tissue and organ formations. So far, analyses of gene function in morphogenesis have mainly focused on the qualitative analysis of phenotypes, often providing limited mechanistic insight into how particular factors act. For this reason, there has been a growing interest in mathematical and computational models to formalize and test hypotheses. These require much more rigorous, quantitative approaches; in parallel, new quantitative and correlative imaging pipelines have been developed to study morphogenesis. Here, we describe a number of such methods, focusing on live imaging.