Analytical Methods for Determining Third and Fourth Generation Fluoroquinolones: A Review.
1The Department of Physical Pharmacy and Pharmacokinetics, Poznań University of Medical Sciences, 6 Swiecickego Street, 60-781 Poznan, Poland.
Chromatographia
|February 21, 2017
Summary
Monitoring third and fourth-generation fluoroquinolones is crucial for effective antibacterial therapy and preventing resistance. This review details analytical methods for determining these vital antibiotic concentrations in various samples.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Microbiology
Background:
- Third and fourth-generation fluoroquinolones exhibit broad-spectrum bactericidal activity.
- Therapeutic drug monitoring of fluoroquinolones is essential for optimizing treatment efficacy and combating antibiotic resistance.
- Key pharmacodynamic parameters like AUC/MIC and Cmax/MIC guide effective fluoroquinolone dosing.
Purpose of the Study:
- To review and summarize analytical methods for determining third and fourth-generation fluoroquinolones.
- To highlight the importance of sample pre-conditioning and mobile phase selection in analytical procedures.
- To provide guidance for selecting appropriate methods for fluoroquinolone determination across different matrices.
Main Methods:
- Review of analytical techniques including chromatography, capillary electrophoresis, and spectroscopy.
- Analysis of various detection methods such as UV, FLD, DAD, and MS.
- Examination of sample pre-conditioning steps like protein precipitation, extraction, filtration, and dilution.
Main Results:
- A comprehensive overview of diverse analytical methods for fluoroquinolone quantification.
- Emphasis on the critical role of sample preparation and mobile phase composition in method performance.
- Identification of various detection techniques coupled with separation methods for accurate drug level determination.
Conclusions:
- Accurate determination of fluoroquinolone levels is vital for effective patient treatment and antimicrobial stewardship.
- The choice of analytical method, including sample preparation and detection, significantly impacts the reliability of fluoroquinolone quantification.
- This review serves as a resource for selecting optimal analytical procedures for fluoroquinolone determination in various biological and pharmaceutical samples.
