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Updated: Mar 7, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
Mapping DNA methylation with high-throughput nanopore sequencing
Arthur C Rand1, Miten Jain1, Jordan M Eizenga1
1Department of Biomolecular Engineering and Genomics Institute, University of California, Santa Cruz, Santa Cruz, California, USA.
Abstract:
DNA chemical modifications regulate genomic function. We present a framework for mapping cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION using this nanopore sequencer's ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in Escherichia coli.
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