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Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
Published on: August 2, 2018
Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen
Hamid Soleimaninejad1, Moore Z Chen1, Xiaoding Lou2
1School of Chemistry, The University of Melbourne, Parkville VIC 3010, Australia. trevoras@unimelb.edu.au.
Abstract:
We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.
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