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Methods to Quantify PAMP-Triggered Oxidative Burst, MAP Kinase Phosphorylation, Gene Expression, and Lignification in
Simon R Lloyd1, Christopher J Ridout1, Henk-Jan Schoonbeek2
1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
None:
Quantitative disease resistance (QDR) based on PAMP-triggered immunity (PTI) could be durable and effective against many pathogens (broad spectrum). Development of methods to evaluate PTI responses in crops could therefore accelerate breeding for durable QDR. Most PTI-studies involved model plants such as Arabidopsis and Nicotiana benthamiana or cell cultures, and cannot be directly applied to diverse germplasm of crop plants.We developed methods to measure PTI in Brassica crop species (Lloyd et al., Mol Plant Microbe Interact 27:286-295, 2014) which we have elaborated and expanded here to enable their use for screening and evaluating germplasm for potential QDR in breeding programs.
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