Related Experiment Video
Updated: Mar 7, 2026
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
A novel quantitative method for recovering precipitated uric acid in urine and analysis by LC-MS/MS
Lifang Sun1, Dongmei Zhou1, David M Wilson1
1Ardea Biosciences, 9390 Towne Centre Drive, San Diego, CA 92121, USA.
Aim:
Precipitation of uric acid (UA) in human urine had caused poor recovery and underestimation of UA in study samples.
Results:
A UA recovery method was developed for analyzing frozen urine samples that potentially contain precipitated UA. Samples were treated with 3% v/v of a solution containing 8 M potassium hydroxide and 20% v/v of ammonium thioglycolate. The high pH enables rapid dissolution of UA crystals, while the thioglycolate protects UA from oxidation at high pH. The treated samples were diluted 20-fold with 50 mM ammonium chloride and analyzed immediately by LC-MS/MS or stored at -20°C prior to analysis.
Conclusion:
Complete recovery of UA was achieved. Thioglycolate stabilized UA at pH 13 for at least 4.5 h at room temperature. Both accuracy and precision satisfy US FDA bioanalytical method validation (BMV) guidelines.
More Related Videos
09:28One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
Published on: June 25, 2010
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021