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Updated: Mar 7, 2026

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
Published on: February 15, 2021
Synthesis of C6''-modified α-C-GalCer analogues as mouse and human iNKT cell agonists
Joren Guillaume1, Toshiyuki Seki2, Tine Decruy3
1Laboratory for Medicinal Chemistry (FFW), Faculty of Pharmaceutical Sciences, UGent, Ottergemsesteenweg 460, B-9000 Ghent, Belgium. serge.vancalenbergh@ugent.be.
Abstract:
α-GalCer analogues that combine known Th1 polarizing C6''-modifications with a C-glycosidic linkage were synthesized. We employed a protecting group strategy that allowed the preparation of both saturated and unsaturated derivatives with variable C6''-substituents. Selected analogues demonstrate promising activity in mice. Interestingly, the introduction of a 6''-O-pyridinylcarbamoyl substituent to α-C-GalCer restores its antigenicity in human iNKT cells.

