Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overprinting with tomographic volumetric additive manufacturing.

Nature communications·2026
Same author

High-efficiency multi-scale holographic volumetric 3D printing with a phase light modulator.

Light, science & applications·2026
Same author

Revisiting deep information propagation: Fractal frontier and finite-size effects.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Revisiting PSF models: Unifying framework and high-performance implementation.

Journal of microscopy·2025
Same author

Perturbative Fourier ptychographic microscopy for fast quantitative phase imaging.

Optics express·2025
Same author

Training of physical neural networks.

Nature·2025
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.8K

Compact lensless phase imager.

Manon Rostykus, Ferréol Soulez, Michael Unser

    Optics Express
    |March 1, 2017
    PubMed
    Summary
    This summary is machine-generated.

    We developed a compact side illumination system for lensless quantitative phase imaging. This method enhances biological cell imaging with a large field of view and unobstructed sample access, simplifying complex setups.

    More Related Videos

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.4K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.3K

    Related Experiment Videos

    Last Updated: Mar 7, 2026

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.8K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.4K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.3K

    Area of Science:

    • Optical Imaging
    • Biomedical Optics
    • Microscopy

    Background:

    • Lensless quantitative phase imaging (QPI) offers high-contrast observation of biological samples over large fields of view (FOV).
    • Existing QPI systems often involve complex, bulky illumination setups that obstruct sample access and limit practical application.
    • The inherent simplicity of lensless QPI is hindered by challenges in illumination system design, particularly for achieving super-resolution or varied illumination angles.

    Purpose of the Study:

    • To propose and demonstrate a novel, compact side illumination system for lensless QPI.
    • To overcome the limitations of conventional illumination methods by reducing system height and providing an unobstructed view.
    • To enable high-contrast imaging of biological samples with a large FOV using a simplified optical setup.

    Main Methods:

    • Development of a compact side illumination system for lensless quantitative phase imaging.
    • Utilizing multiplexed analog holograms to shape illumination and generate multiple (9) illumination angles.
    • Experimental demonstration of the system's performance on phase samples.

    Main Results:

    • The proposed side illumination system reduces the overall height by an order of magnitude compared to conventional setups.
    • The system provides an unobstructed view of the sample, facilitating easier manipulation and observation.
    • Successful experimental imaging of phase samples was achieved with a field of view of approximately 17 mm².

    Conclusions:

    • The developed side illumination technique significantly simplifies and miniaturizes lensless QPI systems.
    • This approach enhances the practicality and potential widespread adoption of QPI for biological imaging.
    • The unobstructed view and large FOV capabilities make it suitable for observing dynamic cellular processes.