Isolation of Mouse Stromal Vascular Cells for Monolayer Culture
Longhua Liu1, Louise D Zheng1, Sarah R Donnelly1
1Department of Human Nutrition, Foods and Exercise, Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech, 1981 Kraft Drive, Blacksburg, VA, 24061, USA.
Abstract:
Positive energy balance contributes to adipose tissue expansion and dysfunction, which accounts largely for obesity and related metabolic disorders. Thermogenic fat can dissipate energy, activation or induction of which may promote energy balance and address the pressing health issues. Recent studies have shown that stromal vascular fraction (SVF) from white adipose tissue (WAT) can develop both white and brown-like adipocyte phenotypes, thus serving as a unique model to study adipogenesis and thermogenesis. Here, we describe a protocol for effective isolation of mouse SVF from WAT, induction of differentiation, and detection of adipogenesis. Success tips for isolation and culture of SVF are also discussed.
