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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
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[Sperm DNA fragmentation: association with semen parameters in young men]
L V Osadchuk1, D A Tataru1,2, N N Kuznetsova3
1Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS, Novosibirsk.
Urologiia (Moscow, Russia : 1999)
|March 2, 2017
Summary
High sperm DNA fragmentation is linked to male infertility. This study found a negative correlation between DNA fragmentation and sperm quality in young men, suggesting SCSA is useful for fertility studies.
Area of Science:
- Reproductive biology
- Andrology
- Genetics
Background:
- Abnormal sperm DNA integrity is a significant risk factor for male infertility.
- Understanding sperm DNA fragmentation is crucial for assessing male reproductive health.
Purpose of the Study:
- To evaluate sperm DNA fragmentation levels in young men from the general population.
- To investigate the association between sperm DNA fragmentation and semen parameters.
Main Methods:
- Sperm DNA fragmentation index (DFI) was measured using the sperm chromatin structure assay (SCSA) via flow cytometry.
- Standard semen parameters including concentration, motility, and morphology were assessed following WHO guidelines.
Main Results:
- In the cohort, 79% had normal DFI (<15%), 12.4% borderline (15-27%), and 8.6% high (≥27%).
- Men with impaired spermatogenesis showed higher DFI (14.53±1.43%) compared to those with normal semen parameters (8.88±0.77%, p<0.05).
- Significant negative correlations were observed between DFI and sperm concentration (r=-0.21), motility (r=-0.41), and normal morphology (r=-0.34).
Conclusions:
- Sperm DNA fragmentation, assessed by SCSA, is negatively associated with key sperm functional parameters.
- SCSA is a viable method for epidemiological studies on male fertility and DNA integrity.
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