Superresolved spatially multiplexed interferometric microscopy.

Area of Science:

• Optical microscopy
• Superresolution imaging

Background:

• Standard microscopes have limited resolution.
• Previous spatially multiplexed interferometric microscopy (SMIM) techniques exist.

Purpose of the Study:

• To implement superresolution capability onto a regular microscope using angular and time multiplexing.
• To update a commercially available non-holographic microscope into a superresolved holographic one.

Main Methods:

• The superresolved spatially multiplexed interferometric microscopy (S2MIM) technique was developed.
• Angular and time multiplexing were used to achieve superresolution.
• The S2MIM technique was validated on an Olympus BX-60 upright microscope with resolution test targets.

Main Results:

• S2MIM successfully implemented superresolution capability onto a regular microscope.
• A commercially available non-holographic microscope was updated into a superresolved holographic microscope.
• Validation confirmed the superresolution performance using resolution test targets.

Conclusions:

• S2MIM provides a method to upgrade standard microscopes for superresolution imaging.
• The technique offers a cost-effective way to achieve holographic superresolution.
• S2MIM advances the capabilities of existing microscopy hardware.