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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
Published on: August 4, 2018
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High-speed adaptive optics line scan confocal retinal imaging for human eye
Jing Lu1, Boyu Gu1, Xiaolin Wang1
1Department of Ophthalmology, School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.
Plos One
|March 4, 2017
Summary
High-speed adaptive optics imaging reduces eye movement artifacts in retinal imaging. This new system achieves cellular resolution at 200 frames per second, significantly improving image clarity for research.
Area of Science:
- Ophthalmology
- Biomedical Engineering
- Optical Imaging
Background:
- Continuous eye movement causes significant intraframe distortion in high-resolution retinal imaging.
- Adaptive optics (AO) systems are crucial for compensating optical aberrations in the human eye.
Purpose of the Study:
- To develop a high-speed AO line scan confocal retinal imaging system.
- To minimize motion artifacts in high-resolution retinal imaging.
Main Methods:
- Employed a high-speed line camera and custom AO to compensate for ocular aberrations.
- Assessed spatial resolution and signal-to-noise ratio in model and human eyes.
- Quantified intra-frame distortion reduction at 30, 100, and 200 frames per second (FPS).
Main Results:
- Achieved cellular-level resolution at 200 FPS with 512x512 pixels/frame in living human eyes.
- Resolved cone and rod photoreceptors in healthy human subjects.
- Reduced intra-frame distortion by 50.9% to 79.7% at 200 FPS compared to 30 FPS.
Conclusions:
- Demonstrated the feasibility of high-resolution retinal imaging at speeds minimizing motion artifacts.
- The developed system can aid research in subjects with nystagmus or unsteady fixation.
