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[Preparation and identification of polyclonal antibody of Streptococcus salivarius 57.I urease]
Yan Wang1, Dan-Ying Tao, Xi-Ping Feng
1Department of Preventive Dentistry, Shanghai Stomatological Hospital. Shanghai 200001, China.
Purpose:
To prepare and identify polyclonal antibody of Streptococcus salivarius 57.I urease.
Methods:
The biggest structural subunit of Streptococcus salivarius 57.I urease, UreC, was obtained by gene clone, IPTG-induced expression, and purification through affinity chromatography. Anti-sera and polyclonal antibody were raised by immunizing rabbits with purified UreC. Western blot was utilized to detect the specific combination of polyclonal antibody with UreC.
Results:
Purified UreC protein was prepared and used as antigen to immunize rabbits. Polyclonal antibody was obtained， and Western blot displayed a specific band of the polyclonal antibody with UreC about 62 kD as anticipated.
Conclusions:
Polyclonal antibody against Streptococcus salivarius 57.I urease is obtained, which provides an important tool to explore the function of urease and its relationship with dental caries.
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