Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

The 5'-CA DNA-sequence preference of daunomycin.

A Skorobogaty¹, R J White, D R Phillips

¹Department of Chemistry, La Trobe University, Bundoora, Victoria, Australia.

|January 25, 1988

Summary

Daunomycin preferentially binds to 5’-CA DNA sequences, suggesting its biological activity stems from interactions with these regulatory elements. This finding aids in understanding daunomycin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

How Well Do We Know the Neutron-Matter Equation of State at the Densities Inside Neutron Stars? A Bayesian Approach with Correlated Uncertainties.

Physical review letters·2020

Same author

Power counting of contact-range currents in effective field theory.

Physical review letters·2015

Same author

Measurement of Compton scattering from the deuteron and an improved extraction of the neutron electromagnetic polarizabilities.

Physical review letters·2015

Same author

Radioarsenic from a portable (72)Se/(72)As generator: a current perspective.

Current radiopharmaceuticals·2012

Same author

Processing of anthracycline-DNA adducts via DNA replication and interstrand crosslink repair pathways.

Biochemical pharmacology·2012

Same author

Barminomycin, a model for the development of new anthracyclines.

Anti-cancer agents in medicinal chemistry·2009

Area of Science:

Molecular Biology
Pharmacology
Genetics

Background:

Daunomycin is an anthracycline antibiotic used in cancer chemotherapy.
Its mechanism of action involves DNA intercalation, but sequence specificity is not fully understood.

Purpose of the Study:

To investigate the DNA sequence specificity of daunomycin binding.
To identify preferred binding sites for daunomycin on DNA.

Main Methods:

DNase I footprinting assays to map DNA-protein interactions.
Escherichia coli RNA polymerase transcription-inhibition assays to assess functional binding.

Main Results:

The 5'-CA sequence was identified as the highest affinity binding site for daunomycin.
Modest affinity sites included 5'-GC, CG, CT, TC, and AC.
Poor affinity sites included 5'-AA, AT, and TA.

Conclusions:

Daunomycin exhibits a strong preference for 5'-CA nucleotide sequences in DNA.
This sequence preference suggests daunomycin's biological activity may involve interactions with eukaryotic genetic regulatory elements containing 5'-CA motifs.

Related Experiment Videos