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Biocatalysis engineering: the big picture.

Roger A Sheldon1, Pedro C Pereira

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Summary
This summary is machine-generated.

This review details a comprehensive strategy for creating and improving enzyme-driven biotransformations. It covers engineering various components for efficient biocatalysis, highlighting economic and environmental advantages.

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Area of Science:

  • Biocatalysis and Enzyme Engineering
  • Green Chemistry
  • Industrial Biotechnology

Background:

  • Applied biocatalysis offers significant economic and environmental advantages over traditional chemical synthesis.
  • There is a growing interest in utilizing isolated enzymes for sustainable chemical transformations.

Purpose of the Study:

  • To present a holistic approach for the invention, development, and optimization of biotransformations using isolated enzymes.
  • To provide a tutorial review on the engineering aspects of applied biocatalysis.

Main Methods:

  • The review discusses engineering of multiple components including substrate, medium, protein (enzyme), and biocatalyst formulation.
  • It also covers engineering of biocatalytic cascades and reactor systems for integrated biotransformation processes.

Main Results:

  • A systematic framework for designing and optimizing enzymatic biotransformations is presented.
  • The review emphasizes the interconnectedness of various engineering components for successful biocatalysis.

Conclusions:

  • A holistic, multi-component engineering strategy is crucial for advancing applied biocatalysis.
  • Optimized biotransformations using isolated enzymes contribute to sustainable and efficient industrial processes.