Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mesopore-Depth Optimization in Mesoporous Gold Films for SF<sub>6</sub> Electroreduction.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Resonant Properties of Chemisorbed Soft Materials on a MHz-Oscillating Solid-liquid Interface.

Journal of oleo science·2026
Same author

Differential and Temporally Dynamic Involvement of Primate Amygdala Nuclei in Face Reality and Reward Information Processing.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

Signal enhancement from nanoparticles using dark-field microscopy with grating-coupled surface plasmon resonance.

The Analyst·2025
Same author

Accurate evaluation of diffusion coefficient for electroactive analytes in human serum samples using nitrogen-terminated sputtered carbon film electrode.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Single-Extracellular-Vesicle Detection with a Plasmonic Chip and Enhanced Fluorescence Microscopy.

ACS omega·2024

Related Experiment Video

Updated: Mar 6, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K

Properties of modified surface for biosensing interface.

Mutsuo Tanaka1, Takahiro Sawaguchi1, Yoshiki Hirata1

  • 1Health Research Institute, Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

Journal of Colloid and Interface Science
|March 14, 2017
PubMed
Summary

Surface modifications using various compounds enhance resistance to salting-out effects and protein adsorption. Alkyl group incorporation improves these properties, offering versatile applications in biomaterials and surface science.

Keywords:
BiosensingMercapto compoundProtein adsorptionSalting-out effectSurface modificationWettability

More Related Videos

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.5K
Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

11.7K

Related Experiment Videos

Last Updated: Mar 6, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K
Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.5K
Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

11.7K

Area of Science:

  • Materials Science
  • Biomaterials Engineering
  • Surface Chemistry

Background:

  • Protein adsorption on surfaces is a significant challenge in biomaterial applications.
  • Developing surfaces with controlled properties like salting-out resistance and wettability is crucial.

Purpose of the Study:

  • To evaluate surface modification strategies for improved resistance to salting-out effects and protein adsorption.
  • To assess the impact of different chemical moieties on surface wettability.

Main Methods:

  • Gold nanoparticles were modified with various mercapto compounds.
  • Salting-out effect resistance was tested using PBS and NaCl solutions.
  • Protein nonspecific adsorption was measured using Surface Plasmon Resonance (SPR).
  • Wettability was quantified on SPR chips.

Main Results:

  • Several modified surfaces, including those with 8C3EG, 12C4EG, 12CPC, 6CCP, and 12CCP, demonstrated excellent salting-out effect resistance.
  • Effective suppression of protein adsorption was observed with 6EG, 12C4EG, 12CPC, and 12CS.
  • Most modified surfaces exhibited high wettability, with M6EG being an exception.
  • Alkyl group incorporation enhanced both salting-out resistance and protein adsorption suppression.

Conclusions:

  • Alkyl group incorporation in surface modifiers is a key strategy for improving salting-out resistance and reducing protein adsorption.
  • Zwitterionic derivatives, particularly inverse phosphoryl choline, show potential for high salting-out resistance and wettability, though with lower protein adsorption suppression.