NicoLase-An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy

Area of Science:

• Optics and Photonics
• Biomedical Imaging
• Open-Source Instrumentation

Background:

• Modern fluorescence microscopy demands versatile, high-power illumination across broad wavelength ranges.
• Existing laser systems can be costly and lack flexibility for diverse super-resolution techniques.

Purpose of the Study:

• To introduce NicoLase, an open-source diode laser combiner, fiber launch, and software controller.
• To provide a flexible and accessible illumination solution for fluorescence and super-resolution microscopy.

Main Methods:

• Designed and built two configurations of the NicoLase system (4 or 6 output wavelengths).
• Integrated multiple diode lasers into a single fiber launch.
• Developed open-source software for sequence control.

Main Results:

• Demonstrated a modular and scalable laser combining system.
• Achieved high power output across a wide spectral range.
• Made all design files and source code publicly available.

Conclusions:

• NicoLase offers a cost-effective and customizable illumination solution for advanced microscopy.
• The open-source nature promotes wider adoption and further development in the research community.
• Enables enhanced capabilities for super-resolution microscopy applications.