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Recent advances in enzyme extraction strategies: A comprehensive review
Shamraja S Nadar1, Rohini G Pawar1, Virendra K Rathod1
1Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
International Journal of Biological Macromolecules
|March 18, 2017
Summary
Industrial enzyme recovery is enhanced by aqueous two-phase systems (ATPS). This review covers traditional, advanced, and integrated ATPS methods for efficient enzyme purification and immobilization.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Separation Science
Background:
- Industrial enzyme production necessitates efficient downstream processing for recovery and purification.
- Existing methods often face challenges in maintaining enzyme activity and scalability.
- Aqueous Two-Phase Systems (ATPS) offer a promising alternative for enzyme recovery.
Purpose of the Study:
- To provide a comprehensive overview of ATPS for enzyme recovery.
- To review traditional, advanced, and integrated ATPS strategies.
- To highlight novel approaches for simultaneous enzyme extraction, purification, and immobilization.
Main Methods:
- Review of polymer/polymer and polymer/salt ATPS, analyzing key parameters (tie line length, pH, salts).
- Exploration of advanced ATPS using alcohols, surfactants, and micellar compounds.
- Examination of integrated ATPS techniques (affinity ligands, ionic liquids, thermoseparating polymers, microfluidics).
- Discussion of three-phase partitioning and CLEAs technology.
Main Results:
- ATPS demonstrates versatility, cost-effectiveness, and scalability for enzyme purification.
- Advanced ATPS and integrated techniques improve selectivity and efficiency.
- Novel strategies like CLEAs and nanoflowers enable simultaneous enzyme processing.
Conclusions:
- ATPS are powerful tools for efficient enzyme recovery and purification.
- Integration of ATPS with other technologies enhances bioseparation efficiency.
- Emerging strategies offer simultaneous extraction, purification, and immobilization, advancing enzyme technology.
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