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Related Experiment Videos

Oxygen radical induced alterations in polyclonal IgG.

H R Griffiths1, J Lunec, C A Gee

  • 1Department of Biochemistry, Selly Oak Hospital, Birmingham, England.

FEBS Letters
|March 28, 1988
PubMed
Summary
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Hydroxyl (OH) radicals cause immunoglobulin G (IgG) aggregation and fluorescence in rheumatoid arthritis. Superoxide radicals are inert, while peroxy radicals cause fluorescence without aggregation, suggesting OH and peroxy radicals may denature IgG in vivo.

Area of Science:

  • Biochemistry
  • Immunology
  • Free Radical Chemistry

Background:

  • Rheumatoid arthritis (RA) patients exhibit aggregated and fluorescent immunoglobulin G (IgG) in sera and synovial fluid.
  • Oxygen-free radicals are suspected contributors to IgG denaturation in RA, but the specific radical species remain unidentified.

Purpose of the Study:

  • To investigate the effects of different oxygen-free radicals on polyclonal IgG.
  • To identify the specific radical species responsible for IgG aggregation and fluorescence.

Main Methods:

  • Polyclonal IgG was exposed to radiolytically generated oxygen-free radicals (hydroxyl, superoxide, and peroxy radicals).
  • Aggregation and fluorescence changes in IgG were monitored.

Main Results:

Related Experiment Videos

  • Hydroxyl (OH) radicals induced both IgG aggregation and a new fluorescence (Ex 360 nm, Em 454 nm).
  • Superoxide radical anions did not induce aggregation or fluorescence.
  • Peroxy radicals induced autofluorescence but not aggregation.

Conclusions:

  • The findings suggest that hydroxyl (OH) radicals are a primary cause of IgG aggregation and fluorescence.
  • Peroxy radicals may contribute to IgG autofluorescence.
  • OH and/or peroxy radical attack represents a potential in vivo mechanism for IgG denaturation in rheumatoid arthritis.