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Related Experiment Video

Updated: Mar 5, 2026

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

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Targeted Ablation Using Laser Nanosurgery.

Naga Venkata Gayathri Vegesna¹, Paolo Ronchi^1,2, Sevi Durdu¹

¹Cell Biology and Cell Biophysics Unit, EMBL Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|March 22, 2017

Summary

This summary is machine-generated.

Advanced laser nanosurgery precisely ablates cellular structures in living cells and multicellular organisms. This powerful technique offers new possibilities for biological research and cellular manipulation with sub-micrometer accuracy.

Keywords:

Laser ablation Laser nanosurgery Micropuncture Photobleach Subcellular structures

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Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Laser-mediated dissection has evolved significantly.
Recent advancements enable sub-micrometer precision for cellular ablation.

Purpose of the Study:

Discuss laser nanosurgery setups and instrumentation.
Describe applications in living cells and multicellular organisms.

Main Methods:

Utilizing pulsed lasers with diffraction-limited precision.
Ablating intracellular structures, including cytoskeletal elements.
Micropuncturing and ablating cells in multicellular organisms.

Main Results:

Demonstrated precise ablation of cytoskeletal elements in living cells.
Showcased applicability in multicellular organisms for targeted cell manipulation.
Established methods for monitoring successful laser nanosurgery.

Conclusions:

Laser nanosurgery provides a precise and versatile tool for cellular research.
The technique facilitates detailed investigation of cellular functions and interactions.