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Updated: Mar 5, 2026
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
Published on: November 13, 2013
From snapshots to movies: Understanding early tooth development in four dimensions
Rebecca Kim1, Jeremy B A Green2, Ophir D Klein1,3,4
1Program in Craniofacial Biology and Department of Orofacial Sciences, University of California San Francisco, San Francisco, California.
Abstract:
The developing tooth offers a model for the study of ectodermal appendage organogenesis. The signaling networks that regulate tooth development have been intensively investigated, but how cell biological responses to signaling pathways regulate dental morphogenesis remains an open question. The increasing use of ex vivo imaging techniques has enabled live tracking of cell behaviors over time in high resolution. While recent studies using these techniques have improved our understanding of tooth morphogenesis, important gaps remain that require additional investigation. In addition, some discrepancies have arisen between recent studies, and resolving these will advance our knowledge of tooth development. Developmental Dynamics 246:442-450, 2016. © 2017 Wiley Periodicals, Inc.
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