Chloroform-free permeabilization for improved detection of β-galactosidase activity in Vibrio cholerae
Charlotte Toulouse1, Claudia C Häse2, Julia Steuber1
1Institute of Microbiology, University of Hohenheim (Stuttgart), 70599 Stuttgart, Germany.
Journal of Microbiological Methods
|March 25, 2017
Abstract:
LacZ (β-galactosidase) is used to monitor the transcription of genes in reporter strains carrying the lacZ gene under the control of a promotor of interest. This protocol for LacZ activity determinations in Vibrio cholerae following detergent lysis results in 2.5-fold increase of LacZ activities compared to lysis with chloroform.


