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Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology.

Marianne Geiser1, Natalie Jeannet2, Martin Fierz3

  • 1Institute of Anatomy, University of Bern, Baltzerstrasse 2, P.O. Box 922, 3012 Bern, Switzerland. marianne.geiser@ana.unibe.ch.

Nanomaterials (Basel, Switzerland)
|March 25, 2017
PubMed
Summary
This summary is machine-generated.

New nano aerosol chamber for in vitro toxicity (NACIVT) enables realistic inhalation safety testing. Exposure to silver and carbon nanoparticles showed similar, small cellular responses in airway epithelia, with increased cytokine release at higher doses.

Keywords:
3RNACIVTaerosolair-liquid interfaceairway epitheliaelectrostatic depositionengineered nanoparticlesin vitronanoparticlestoxicology

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Area of Science:

  • Nanotoxicology
  • Inhalation Toxicology
  • In Vitro Safety Testing

Background:

  • Increasing use of nanoparticle (NP)-containing products poses risks of incidental exposure, particularly in occupational settings and for consumers using NP sprays.
  • Existing in vitro methods may not fully replicate realistic inhalation exposure scenarios for diverse populations.
  • Silver (Ag) and carbon (C) nanoparticles are widely used, necessitating robust safety assessments.

Purpose of the Study:

  • To develop and evaluate the nano aerosol chamber for in vitro toxicity (NACIVT) as a portable instrument for realistic inhalation safety testing of NPs.
  • To assess the effects of silver and carbon nanoparticles on normal and compromised airway epithelia using the NACIVT system.
  • To compare the NACIVT system's performance and suitability for short and long-term exposures with air-liquid interface (ALI) cell cultures against existing systems.

Main Methods:

  • Development of the portable nano aerosol chamber for in vitro toxicity (NACIVT) instrument.
  • Exposure of normal and compromised airway epithelia (ALI cell cultures) to silver (Ag) and carbon (C) nanoparticles using NACIVT.
  • Evaluation of NP effects on cellular responses and cytokine release.
  • Review of NACIVT's physical performance, suitability for ALI cultures, and comparison with other commercial systems.

Main Results:

  • A single exposure to realistic doses of spark-generated 20-nm Ag or C nanoparticles resulted in small, comparable cellular responses for both NP types.
  • Cytokine release from airway epithelia generally increased with increasing nanoparticle dose.
  • The NACIVT system demonstrated suitability for realistic in vitro inhalation toxicology testing.

Conclusions:

  • The developed NACIVT system provides a realistic in vitro approach for assessing inhaled nanoparticle toxicity.
  • Silver and carbon nanoparticles elicit similar, dose-dependent cellular responses in airway epithelia.
  • Further research using NACIVT can inform safety guidelines for nanoparticle-containing products.