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End-Modifications on Morpholino Oligos.

Yong-Fu Li1

  • 1Gene Tools, LLC, 1001 Summerton Way, Philomath, OR, 97370, USA. yli@gene-tools.com.

Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2017
PubMed
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Morpholino oligos can be modified at various positions, enabling conjugation with other molecules via amino groups. Double functionalization at the 3'-end allows for advanced applications like fluorescent detection and in vivo studies.

Area of Science:

  • Chemical Biology
  • Oligonucleotide Chemistry

Background:

  • Morpholino oligos are versatile nucleic acid analogs.
  • Functionalization of oligos is key for diverse applications.

Purpose of the Study:

  • To explore modifications of Morpholino oligos for conjugation.
  • To develop methods for advanced 3'-end double functionalization.
  • To enable custom molecule assembly for specific applications.

Main Methods:

  • Chemical modification of Morpholino oligos at ends and internal positions.
  • Utilizing amino groups as reactive entities for conjugation.
  • Employing orthogonally protected diamine for solid-phase synthesis.
  • Developing double modification strategies at the 3'-end.
Keywords:
Amino groupsConjugationEnd-modificationFunctionalizationMorpholino oligoVis-Vivo-Morpholino

Related Experiment Videos

Main Results:

  • Demonstrated that modifications at either end, both ends, or internally provide functional groups for conjugation.
  • Identified amino groups as efficient reactive sites for chemical bonding.
  • Showcased advanced 3'-end double modification using a protected diamine.
  • Facilitated the assembly of custom-designed molecules.

Conclusions:

  • Morpholino oligo modifications offer extensive opportunities for conjugation and custom molecule synthesis.
  • 3'-end double functionalization enables sophisticated applications, such as Vis-Vivo-Morpholino.
  • This approach supports fluorescent detection and in vivo intracellular activity studies.