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Fowl Adenovirus-Based Vaccine Platform
Juan C Corredor1, Yanlong Pei1, Éva Nagy2
1Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON, Canada, N1G 2W1.
Methods in Molecular Biology (Clifton, N.J.)
|April 5, 2017
Summary
Nonpathogenic fowl adenoviruses (FAdVs) can be engineered into multivalent vaccine platforms. Researchers successfully created FAdV-9 vaccine platforms using homologous recombination for foreign gene expression.
Area of Science:
- Veterinary Virology
- Molecular Biology
- Vaccine Development
Background:
- Nonpathogenic fowl adenoviruses (FAdVs) possess large nonessential genomic regions, making them suitable for genetic engineering.
- These characteristics allow for the development of novel vaccine platforms.
Purpose of the Study:
- To engineer FAdV-9-based vaccine platforms for potential multivalent vaccine applications.
- To demonstrate the feasibility of inserting foreign genes into the FAdV-9 genome for expression.
Main Methods:
- Utilized targeted homologous recombination within an infectious clone (pPacFAdV-9) of the FAdV-9 genome.
- Released viral DNA from a cosmid vector via restriction enzyme digestion.
- Transfected the viral DNA into a chicken hepatoma cell line (CH-SAH) for virus propagation.
Main Results:
- Successfully generated FAdV-9-based vaccine platforms.
- Confirmed the propagation of the engineered virus.
- Verified the expression of foreign genes within the FAdV-9 platform.
Conclusions:
- FAdV-9 serves as a viable platform for developing multivalent vaccines.
- Homologous recombination is an effective method for engineering FAdV genomes.
- The engineered FAdV-9 platform can express foreign genes, supporting its use in vaccine development.