Renal handling of nourseothricin

Nourseothricin 1 is preferentially excreted via kidney and signs of nephrotoxicity can be observed after its administration. Renal handling of 1 was characterized in experiments on renal cortical slices under various experimental conditions. Following administration in vivo or in vitro the renal tubular transport system for organic anions (p-aminohippurate, PAH) is not influenced by 1. There is a high degree of accumulation of 1 in renal cortical slices. In contrast to PAH accumulation there is no influence of nitrogen atmosphere, simultaneous administration of PAH, probenecid or trishydroxyaminomethane on 1 accumulation. Age dependent differences in 1 accumulation does not exist. Our data show a distinct uptake of 1 in kidney tissue. Accumulation of 1 in renal cortical slices is caused by binding; an active tubular transport of 1 in renal cortical slices could be excluded.